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KMID : 1041219910330010021
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Journal of Sericultural and Entomological Science
1991 Volume.33 No. 1 p.21 ~ p.26
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Polyhedral Protein Synthesis and DNA Replication of Bombyx mori, Nuclear Polyhedrosis Virus in a B. mori Cell Line
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陳炳來/Jin, Byung Rae
朴範錫/柳江善/諸蓮鎬/姜錫權/Park, Beom Seok/Ryu, Kang Sun/Je, Yeon Ho/Kang, Seok Kwon
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Abstract
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Bombyx mori nuclear polyhedrosis virus (BmNPV) was successfully multiplied in the nuclei of BmN4 cells cultured with insect Grace´s medium. By electron microscopic observation, the virons had a single nucleocapsid in an envelope. Polyhedral protein synthesis of BmNPV in BmN4 cells was detected at 18 hr p.i. and polyhedral protein was a single-polypeptide with a M.W of 30 kd. At 48 hr p.i. polyhedra formation was observed by inverted microscope and electron microscope. Genome analysis of BmNPV by restriction endonucleases was not revealed the difference between virus produced in vivo and that in vitro.
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